Sample number and denaturation time are crucial for the accuracy of capillary-based LightCyclers.

changes observed on electron microscopy, and the mtDNA mutations. The electron microscopy study of peptic ulcer tissue showed swollen mitochondria and a loss of cristae within the mitochondrial cytoplasm. The mtDNA copy number of the peptic ulcer tissue samples was 2.5 times higher than that from the blood cells (2 180 547 vs 863 846 copies/ L). Proposed interactions among host, environment, and H. pylori infection are different in the development of gastric and duodenal ulcers. However, ROS are commonly released in the inflamed gastroduodenal mucosa caused by H. pylori infection after ulcer development. In our limited number of patients we did not observe any significant differences between gastric and duodenal ulcers for ROS level in the supernatants or the frequency and pattern of mtDNA alterations.